How do I prepare my templates for the high-volume sequencing service on the ABI3730xl?
Templates are brought to the facility in 96-well format.
- If using more than one primer on the plate, please provide a plate with primers in the wells corresponding to the proper template locations.
- DNA must be of high purity, and consistent concentration for best results. The 3730xl sequencer is very sensitive to salts.
- Users wishing to use the facility's automated sample cleanup, quantification, and normalization services should submit their plates to those services before sequencing.
- Optimum concentration for PCR products is 10ng/ul & for plasmids 50ng/ul. Please supply at least 8ul of template for each reaction to be performed.
- Optimum primer concentration 3 pmoles/ul. Please supply at least 4ul of primer for each reaction to be performed.
